Modern life science and biotech applications require the detection of biocatalytical activity of a specific enzyme in live cells without the use of antibodies. However, today’s chromogenic substrates suffer from low performance and marketed fluorogenic substrates lack robustness. The use of enzyme-responsive probes releasing a precipitated signal can meet the significant challenges, i.e. sensitivity, fast response time, and signal retention, needed by industrials players working on the detection of microorganisms, in situ cancer diagnosis, or high-throughput enzyme assays.
Many reports of the past 20 years have highlighted the unique properties of the solid-state fluorophore HPQ, also known as “ELF 97 alcohol”. HPQ is a highly attractive candidate for the incorporation into enzyme-responsive molecular probes that furnish high sensitivity, rapid response, and signal retention. But HPQ has unfavorable chemical properties with regard to its incorporation into stable and robust molecular probes. Hence, a single probe has hitherto been marketed, responding to generic phosphatase activity. It is here that Molsid’s innovative chemical technology changes the game by offering robust probes for many enzyme activities.